Erythroid Progenitor Cells Expanded from Peripheral Blood without Mobilization or Preselection: Molecular Characteristics and Functional Competence

Filippone Claudia, Franssila Rauli, Kumar Arun, Saikko Leena, Kovanen Panu E., Söderlund-Venermo Maria, Hedman Klaus
PLoS ONE, 2010-03-02; 5(3), e9496
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Reviews and questions

Perfectly reproducible

by Racoon, 30 Jun 07:21

Even though the general idea in the field is still that one needs to select for CD34+-cells for ex vivo erythropoeisis, this paper shows that this is not necessary. I've used this protocol myself (with minor changes, e.g. different medium) and have successfully obtained reticulocytes.

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